The isolation and identification of the B protein of lactose synthetase as alpha-lactalbumin.

نویسندگان

  • U Brodbeck
  • W L Denton
  • N Tanahashi
  • K E Ebner
چکیده

The B protein, a subunit of lactose synthetase (EC 2.4.1. c), was crystallized from bovine skim milk and bovine mammary tissue. The B protein was identified as cu-lactalbumin, based on the following criteria: substitution in the enxymic rate assays, spectra, immunological titrations, amino acid composition, mobility on starch gel electrophoresis, molecular weight, and cochromatography on diethylaminoethyl cellulose and Sephadex G-100. Thus the biological function of cr-ladalbumin is as a natural occurring subunit of lactose synthetase.

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منابع مشابه

The Isolation and Identification of the B Protein of Lactose Synthetase as a-Lactalbumin*

The B protein, a subunit of lactose synthetase (EC 2.4.1. c), was crystallized from bovine skim milk and bovine mammary tissue. The B protein was identified as cu-lactalbumin, based on the following criteria: substitution in the enxymic rate assays, spectra, immunological titrations, amino acid composition, mobility on starch gel electrophoresis, molecular weight, and cochromatography on diethy...

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The lactose synthetase particles of lactating bovine mammary gland. Preparation of particles with intact lactose synthetase.

1. The particulate form of lactating bovine mammary lactose synthetase activity is shown to be more highly organized than previously reported. 2. A novel method of shattering frozen mammary tissue with effective cell disruption is described. 3. The apparent subcellular distribution of lactose synthetase was shown to reflect the method of homogenization. 4. After mild homogenization particles as...

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Progesterone and the metabolic control of the lactose biosynthetic pathway during lactogenesis in the rat.

1. Lactogenesis was initiated in pregnant rats by ovariectomy, thereby causing progesterone withdrawal, after which the mammary tissue was analysed for contents of enzymes and metabolites concerned with the biosynthesis of lactose. 2. Lactose synthesis increased about 126-fold with little or no accompanying change in the contents of most metabolic intermediates or in the adenine nucleotide ener...

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 242 7  شماره 

صفحات  -

تاریخ انتشار 1967